| Mycoplasma species Reagent Set |
|
| Reagent Set for the detection of Mycoplasma in cell culture supernatant |
Mycoplasma
can easily contaminate cell cultures. Traditional detection methods (cultural
and immunoenzymatic often do not have sufficient sensitivity to detect low
grade contaminations) The primers are able to detect all Mycoplasma species
and allow a high sensitivity |
|
| Starting sample |
200µl of growing medium |
|
| Primers |
Primers
are aliquoted in single reaction tubes and isolated by paraffin (HOT-START)
Primers contain an UV-activable DNA cross-linker that makes the amplicons
not further suitable for PCR (post-amplification decontamination) |
|
| Reagents supplied |
Sixty
tubes containing 20µl of primer solution includes also dNTPs and Thermostable
DNA polymerase (red vials). Two tubes containing 60µl of positive control
including also dNTPs and Thermostable DNA polymerase (green vials) Each tube
contains all the reagents required to start with the amplification procedure.
After the addition of the sample do not add polymerase or dNTPs! Before opening
the tubes, lay them down on a transilluminator and expose the DNA amplicons
to UV light for 60 seconds. The PCR fragments, UV activated, will not be
amplified anymore. Open the tubes, and perform agarose electrophoresis |
|
| Interpretation of the results |
Samples are considered positive when after electrophoresis a band of 700bp is detected. |
|
| Storage and stability |
• Twelve months at -20°C |
